Purification and activity assurance of precipitated heterologous

Details Purification and activity assurance of precipitated heterologous Purification and activity assurance of precipitated heterologous

1984-06-01

1985-04-16

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

424 85, 435 68, 435 70, 4351723, 435240, 435241, 435948, 436548, 935 60, C07G 700

Patent

active

045115028

ABSTRACT:
A process for purifying heterologous proteins from higher molecular weight components, including dissolving the heterologous protein in a strong denaturing solution and removing the higher molecular weight components using a molecular sieve or high speed centrifugation.

REFERENCES:
patent: 4414150 (1983-11-01), Goeddel
patent: 4438032 (1984-03-01), Golde et al.
patent: 4462940 (1984-07-01), Hanisch et al.
Williams et al., Science 215, 687-688, (1982).
Goeddel et al., Proc. Natl. Acad. Sci. USA 76, 106-110, (1979).
Wetzel et al., Biochemistry 19, 6096-6104, (1980).
Wetzel et al., Gene 16, 63-71, (1981).
Ullman et al., Biochem. Biophys. Res. Commun. 35, 35-42, (1969).
Goeddel et al., Nature, vol. 281, pp. 544 to 548, (Oct. 18, 1979).
Kleid et al., Science, vol. 214, pp. 1125 to 1129, (Dec. 4, 1981).

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