Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Chemical modification or the reaction product thereof – e.g.,...
Patent
1984-06-01
1986-07-08
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Chemical modification or the reaction product thereof, e.g.,...
435 68, 435 70, 435240, 435948, 436548, 530406, 530416, 530417, 530825, C07G 320, C07G 322
Patent
active
045991979
ABSTRACT:
A heterologous expression product in host cells is separated from the host cell material by suspending the material in a buffered solution, disrupting the cells and separating the product as refractile material. The refractile material can be dissolved in a strongly denaturing solution, which is then weakened while keeping the protein in solution, thereby allowing unfolding and refolding of the protein.
REFERENCES:
patent: 4414150 (1983-11-01), Goeddel
Williams et al., Science 215, 687-688 (1982).
Goeddel et al., Proc. Natl. Acad. Sci. USA 76, 106-110 (1979).
Wetzel et al., Biochemistry 19, 6096-6104 (1980).
Wetzel et al., Gene 16, 63-71 (1981).
Ullmann et al., Biochem. Biophys. Res. Commun. 35, 35-42 (1969).
Goeddel et al, Nature, vol. 281, pp. 544 to 548 (Oct. 18, 1979).
Kleid et al., Science, vol. 214, pp. 1125 to 1129 (Dec. 4, 1981).
Genentech Inc.
Schain Howard E.
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