Purification and activity assurance of precipitated heterologous

Details Purification and activity assurance of precipitated heterologous Purification and activity assurance of precipitated heterologous

1984-06-01

1986-11-04

Schain, Howard E.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

435 68, 435 70, 435240, 436548, C08G 312

Patent

active

046209484

ABSTRACT:
A process for isolating and purifying refractile proteins from host cell culture including treating the host cell culture with a buffered solution to solubilize host protein, leaving refractile protein insoluble, treating the insoluble fraction with a strongly denaturing solution containing a reducing agent to solubilize the refractile protein, and renaturing the refractile protein.

REFERENCES:
patent: 4414150 (1983-11-01), Goeddel
patent: 4438032 (1984-03-01), Golde et al.
patent: 4462940 (1984-07-01), Hanisch et al.
Williams et al., Science 215, 687-688 (1982).
Goeddel et al., Proc. Natl. Acad. Sci. USA 76, 106-110 (1979).
Wetzel et al., Biochemistry 19, 6096-6104 (1980).
Wetzel et al., Gene 16, 63-71 (1981).
Ullmann et al., Biochem. Biophys. Res. Commun. 35, 35-42 (1969).
Goeddel et al., Nature, vol. 281, pp. 544 to 548 (Oct. 18, 1979).
Kleid et al., Science, vol. 214, pp. 1125 to 1129 (Dec. 4, 1981).

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