Purification and activation of proteins from insoluble inclusion

Details Purification and activation of proteins from insoluble inclusion Purification and activation of proteins from insoluble inclusion

1985-08-19

1988-08-23

Kight, John

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530416, 530417, 530422, 530399, 530825, 435 68, C07K 328

Patent

active

047662247

ABSTRACT:
Purification and solubilization of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies is effected by solubilizing the inclusion bodies in SDS; treating the SDS-protein solution with urea; removing the SDS and purifying the protein by chromatography on an anion-exchange resin having cationic groups attached to a polysaccharide support; and dialyzing the solution obtained from the anion-exchange resin to remove urea, thereby allowing the protein to fold into its native conformation. The solution thus obtained can be activated by removing soluble protein aggregates via ultrafiltration or chromatography on a weak anion-exchange column.

REFERENCES:
patent: 4426323 (1984-01-01), Jain
patent: 4462940 (1984-07-01), Hanisch et al.
patent: 4511502 (1985-04-01), Builder
patent: 4511503 (1985-04-01), Olson
patent: 4518526 (1985-08-01), Olson et al.
patent: 4599197 (1986-07-01), Wetzel
patent: 4677196 (1987-06-01), Rausch
Protein Purification, Ed. Scopes, Ion Exchange Chromatograph, 1982, pp. 81-82.

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