Purification and activation of proteins from insoluble inclusion

Details Purification and activation of proteins from insoluble inclusion Purification and activation of proteins from insoluble inclusion

1985-09-06

1987-06-30

Kight, John

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530416, 530417, 530422, 530820, 435 68, 435 70, C07K 320, C12P 2100

Patent

active

046771964

ABSTRACT:
Purification and activation of proteins produced in transformant microorganisms as insoluble, biologically inactive inclusion bodies can be effected by solubilizing the inclusion bodies in SDS; dialyzing or refrigerating the protein solution to remove a portion of the SDS; chromatographing the protein solution on an ion-retardation resin to remove the remaining SDS from the protein; and chromatographing the protein solution thus obtained on an anion-exchange resin.

REFERENCES:
patent: 4426323 (1984-01-01), Jain
patent: 4511502 (1985-04-01), Builder
patent: 4511503 (1985-04-01), Alson
patent: 4518526 (1985-05-01), Olson et al.
Kapp et al., Anal. Biochem. 91, 1978, pp. 230-233.

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