Purificaiton procedure of tPA from crude preparations

Details Purificaiton procedure of tPA from crude preparations Purificaiton procedure of tPA from crude preparations

1990-10-24

1992-05-05

Patterson, Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435212, 435219, C12N 964

Patent

active

051107368

ABSTRACT:
Crude tPA containing, as an impurity, a protein which is reactive with an anti-human tPA antibody and has a molecular weight of 110,000.+-.20,000 daltons is purified by bringing the crude tPA into close contact with an affinity reagent containing an immobilized Kunitz inhibitor which is produced in seeds of Erythrina latissima and other Erythrina plants and acts as an inhibitor on trypsin, plasmin and tPA but does not act on urokinase, so that tPA is collected selectively.

REFERENCES:
Chemical Abstracts, vol. 102, No. 1, Jan. 7, 1985, p. 238 Abstract No. 2474f, C. Heussen et al "Purification of Human Tissue . . . ".
Kluft, C. et al., (1983) Advances in Biotechnological Processes, vol. 2, pp. 97-110, A. R. Liss, Inc, N.Y. (Mizrahi and van Wezel, editors).

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