Chemistry: analytical and immunological testing – Including sample preparation – Liberation or purification of sample or separation of...
Patent
1995-12-04
1996-09-24
Snay, Jeffrey
Chemistry: analytical and immunological testing
Including sample preparation
Liberation or purification of sample or separation of...
435 2, 4353081, 210787, B01D 2126
Patent
active
055590403
ABSTRACT:
Pulsing and oscillating methods of centrifuging viable cell samples for improving the quantity and quality of the recovered cells are disclosed. In the pulsing method, the relative centrifugal force applied to the viable cells is pulsed between a predetermined centrifugal force and a negligible or zero centrifugal force by alternately rotating and stopping the centrifuge. The steps of rotating and stopping the centrifuge are alternately repeated until at least three total rotating steps have been completed, preferably for a total rotation time which does not exceed approximately 5 minutes. In the oscillating method, the relative centrifugal force applied to the viable cell samples is oscillated between a relatively high centrifugal force and a relatively low centrifugal force until at least three steps of rotation at the high g force are completed, preferably for a total rotation time which does not exceed approximately 5 minutes. It has been found that the pulsing and oscillating methods yield a significantly higher number of viable cells with better quality than prior art centrifuging methods.
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