Pulsed oriented electrophoresis

Details Pulsed oriented electrophoresis Pulsed oriented electrophoresis

1992-05-04

1995-04-11

Niebling, John

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

2041828, G01N 2726, G01N 27447

Patent

active

054055199

ABSTRACT:
Disclosed herein is a method of electrophoretically separating particles by the application to pulsed field electrophoresis of a pulsing routine in which the pulses are modulated in such a way as to define the effective field angle of the particles undergoing separation as determined by the vector sum of the pulses. In one embodiment of the invention, pulse times are modulated to define the orientation of the particles undergoing separation. In a second embodiment, the intensities of the pulses are modulated to define the orientation of the particles. The method of the invention allows the simple and dynamic determination of effective field angle by pulsing routine rather than by placement of an electrode array. In addition, the invention makes it possible for the first time to translate particles undergoing separation incrementally through a gel matrix.

REFERENCES:
patent: 4473452 (1984-09-01), Cantor et al.
patent: 4737251 (1988-04-01), Carle et al.
patent: 5084157 (1992-01-01), Clark et al.
McPeek, Jr., F. D. "Separation of Large DNA Molecules by Modified Pulsed Field Gradient Gel Electrophoresis" Analytical Biochemistry 156 (1986) 274-285.
Schwartz, et al., "Conformational Dynamics of Individual DNA Molecules During Gel Electrophoesis", Nature, Apr. 6, 1989, pp. 520-522.

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