Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1989-05-05
1992-08-04
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
204299R, G01N 2726, B01D 5702
Patent
active
051356287
ABSTRACT:
An electrophoresis apparatus 10 has a power supply 12, an electrophoresis gel system including the tank 85 and an adjustable switching means 42 connected together for controlling the electric field force, electric field angle and the pulse duration to resolve DNA molecules greater than 1,000 kb in length along straight, unbent lanes within a gel. The electrophoresis system 10 permits adjustment of the pulse durations through a range from generally one-tenth of a second to shorter ranges. It permits the adjustability of the electric field force, electric field angle and the pulse duration so that it may have: (1) pulse durations in a range that maintains the pulse durations shorter than one-tenth of a second; (2) an angle or angles of two fields up to 180 degrees with respect to each other; (3) a side range of intensities of the fields; and (4) any number of repetitions of pulses of the fields before changing the angle of the two fields with respect to each other.
REFERENCES:
Steven M. Clark et al. "Novel Instrument for Separating Large DNA Molecules with Pulsed Homogeneous Electric Fields" Science, 241: 1203-1205 (1988).
Chantal Turmel, "Molecular detrapping and band narrowing with high frequency modulation of pulsed field electrophoresis" Nucleic Acids Research, 18(3) [1990] 569-575.
Gilles Roy et al. "Inexpensive and simple set-up for field inversion gel electrophoresis" Nucleic Acids Research 16(3) [1988] 768.
C. D. Porter et al., "BBC microcomputer controlled field inversion gel electrophoresis" Computer applications in the biosciences:CABIOS, 4(2) [1988] 271-273.
Carney Vincent L.
Isco, Inc.
Niebling John
Starsiak Jr. John S.
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