Pulsed field capillary electrophoresis

Details Pulsed field capillary electrophoresis Pulsed field capillary electrophoresis

1990-05-18

1992-06-16

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, 2041801, C25B 100, C25B 700, B01D 6142

Patent

active

051222485

ABSTRACT:
Apparatus and method for conducting electrophoresis in a capillary tube under the influence of a pulsed electric field. Apparatus for separating and detecting molecular species of a sample in a conductive medium includes a capillary tube for containing conductive medium, means for introducing a sample into a conductive medium-filled capillary tube, means for applying a pulsed electric field across the conductive medium-filled capillary tube between its ends, and means for detecting constituent molecular species of a sample in a conductive medium-filled capillary tube as they traverse the tube. A method for separating and detecting molecular species of a sample in a conductive medium includes the steps of introducing the sample into a capillary tube filled with conductive medium, applying a pulsed electric field of up to 500 volts/centimeter between the ends of the capillary, and detecting the constituent molecular species of the sample as they traverse the capillary.

REFERENCES:
patent: 4865706 (1989-09-01), Karger
patent: 4909919 (1990-03-01), Morris
patent: 4911807 (1990-03-01), Burd
patent: 4985128 (1991-01-01), Ebersole
patent: 5015350 (1991-05-01), Wiktorowicz
Avdalovic, N. and Furst, A.: "An instrument for separating fragments of chromosomal DNA", American Biotech Laboratory, 26-34, May (1989).
Cantor, C., Smith, C. and Mathew, M.: "Pulsed-field gel Electrophoresis of very large DNA molecules", Ann. Rev. Biophys. Biophys. Chem. 17; 287 (1988).
Dawkins, H. J. S.: "Large DNA separation using field alternation agar gel electrophoresis", Journal of Chromatography, 492 (1989) 615-639, Elsevier Science Publishers B.V., Amsterdam.

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