PUFA polyketide synthase systems and uses thereof

Details PUFA polyketide synthase systems and uses thereof PUFA polyketide synthase systems and uses thereof

2011-03-01

2011-03-01

McElwain, Elizabeth F (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Plant, seedling, plant seed, or plant part, per se

Higher plant, seedling, plant seed, or plant part

C800S281000, C435S468000, C435S471000, C435S419000, C435S252300, C536S023200

Reexamination Certificate

active

07897844

ABSTRACT:
Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems fromSchizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

REFERENCES:
patent: 5130242 (1992-07-01), Barclay et al.
patent: 5246841 (1993-09-01), Yazawa et al.
patent: 5639790 (1997-06-01), Voelker et al.
patent: 5672491 (1997-09-01), Khosla et al.
patent: 5683898 (1997-11-01), Yazawa et al.
patent: 5798259 (1998-08-01), Yazawa et al.
patent: 5908622 (1999-06-01), Barclay
patent: 6033883 (2000-03-01), Barr et al.
patent: 6140486 (2000-10-01), Facciotti et al.
patent: 6503706 (2003-01-01), Abken et al.
patent: 6566583 (2003-05-01), Facciotti et al.
patent: 6677145 (2004-01-01), Mukerji et al.
patent: 7001772 (2006-02-01), Roessler et al.
patent: 7087432 (2006-08-01), Qiu et al.
patent: 7125672 (2006-10-01), Picataggio et al.
patent: 7208590 (2007-04-01), Mukerji et al.
patent: 7211418 (2007-05-01), Metz
patent: 7217856 (2007-05-01), Weaver
patent: 7259295 (2007-08-01), Metz et al.
patent: 7271315 (2007-09-01), Metz et al.
patent: 2002/0194641 (2002-12-01), Metz
patent: 2003/0101486 (2003-05-01), Facciotti
patent: 2004/0005672 (2004-01-01), Santi et al.
patent: 2004/0010817 (2004-01-01), Shockey et al.
patent: 2004/0139498 (2004-07-01), Jaworski et al.
patent: 2004/0172682 (2004-09-01), Kinney et al.
patent: 2005/0089865 (2005-04-01), Napier et al.
patent: 2005/0164192 (2005-07-01), Graham et al.
patent: 2005/0266440 (2005-12-01), Metz
patent: 2005/0273883 (2005-12-01), Metz
patent: 2005/0273884 (2005-12-01), Metz
patent: 2007/0244192 (2007-10-01), Metz
patent: 2007/0245431 (2007-10-01), Metz et al.
patent: 2007/0256146 (2007-11-01), Metz et al.
patent: 2007/0266455 (2007-11-01), Weaver et al.
patent: 2007/0270494 (2007-11-01), Metz et al.
patent: 2008/0022422 (2008-01-01), Weaver et al.
patent: 2008/0026434 (2008-01-01), Weaver et al.
patent: 2008/0026435 (2008-01-01), Weaver et al.
patent: 2008/0026436 (2008-01-01), Weaver et al.
patent: 2008/0026437 (2008-01-01), Weaver et al.
patent: 2008/0026438 (2008-01-01), Metz et al.
patent: 2008/0026439 (2008-01-01), Metz et al.
patent: 2008/0026440 (2008-01-01), Metz et al.
patent: 2008/0032296 (2008-02-01), Weaver et al.
patent: 2008/0032338 (2008-02-01), Weaver et al.
patent: 2008/0032351 (2008-02-01), Metz et al.
patent: 2008/0032367 (2008-02-01), Weaver et al.
patent: 2008/0032368 (2008-02-01), Weaver et al.
patent: 2008/0032369 (2008-02-01), Weaver et al.
patent: 2008/0038378 (2008-02-01), Metz et al.
patent: 2008/0038379 (2008-02-01), Metz et al.
patent: 2008/0038790 (2008-02-01), Metz et al.
patent: 2008/0038791 (2008-02-01), Metz et al.
patent: 2008/0038792 (2008-02-01), Metz et al.
patent: 2008/0038793 (2008-02-01), Metz et al.
patent: 2008/0038794 (2008-02-01), Metz et al.
patent: 2008/0038795 (2008-02-01), Metz et al.
patent: 2008/0038796 (2008-02-01), Metz et al.
patent: 2008/0038797 (2008-02-01), Metz et al.
patent: 2008/0038798 (2008-02-01), Weaver et al.
patent: 2008/0038799 (2008-02-01), Weaver et al.
patent: 2008/0040822 (2008-02-01), Metz et al.
patent: 2008/0044867 (2008-02-01), Metz et al.
patent: 2008/0044868 (2008-02-01), Metz et al.
patent: 2008/0044869 (2008-02-01), Metz et al.
patent: 2008/0044870 (2008-02-01), Metz et al.
patent: 2008/0044871 (2008-02-01), Metz et al.
patent: 2008/0044872 (2008-02-01), Metz et al.
patent: 2008/0044873 (2008-02-01), Metz et al.
patent: 2008/0044874 (2008-02-01), Weaver et al.
patent: 2008/0050790 (2008-02-01), Metz et al.
patent: 2008/0050791 (2008-02-01), Weaver et al.
patent: 2520795 (2004-10-01), None
patent: 0594868 (1994-05-01), None
patent: 0823475 (1998-02-01), None
patent: 9323545 (1993-11-01), None
patent: 9621735 (1996-07-01), None
patent: 9846764 (1998-10-01), None
patent: 9855625 (1998-12-01), None
patent: 0042195 (2000-07-01), None
patent: WO 02/083870 (2002-10-01), None
patent: WO 2004/087879 (2004-10-01), None
patent: WO 2006/008099 (2006-01-01), None
patent: WO 2006/034228 (2006-03-01), None
U.S. Appl. No. 11/674,574, filed Feb. 13, 2007, Facciotti et al.
U.S. Appl. No. 11/777,277, filed Jul. 12, 2007, Metz et al.
U.S. Appl. No. 11/778,594, filed Jul. 16, 2007, Metz et al.
U.S. Appl. No. 11/781,861, filed Jul. 23, 2007, Weaver et al.
U.S. Appl. No. 11/781,882, filed Jul. 23, 2007, Weaver et al.
Allen E.A. et al. 2002 “Structure and regulation of the omega-3 polyunsaturated fatty acid synthase genes from the deep-sea bacteriumPhotobacterium profundumstrain SS9” Microbiology vol. 148 pp. 1903-1913.
Cane et al., “Harnessing the Biosynthetic Code: Combinations, Permutations, and Mutations.” Science 1998, vol. 282, pp. 63-68.
GenBank Accession No. AF4091 00, (Allen et al.) 2002.
Kaulmann et al. “Biosynthesis of Polyunsaturated Fatty Acids by Polyketide Synthases”, Angew. Chem. Int. Ed. 2002, 41, No. 11, pp. 1866-1869.
Khosla et al., “Tolerance and Specificity of Polyketide Synthases”, Annu. Rev. Biochem. 1999. 68:219-253.
Nakahara et al. Production of docosahexaenoic and docosapentaenoic acids bySchizochytriumsp. isloated from Yap Islands. 1996 J. Am. Oil Chem. Soc. 1996, vol. 73, No. 11, pp. 1421-1426.
Napier “Plumbing the depths of PUFA biosynthesis: a novel polyketide synthase-like pathway from marine organisms.” Trends in Plant Science. Feb. 2002, vol. 7, No. 2, pp. 51-54.
Nasu et al., “Efficient Transformation ofMarchantia polymorphaThat is Haploid and Has Very Small Genome DNA,” Journal of Fermentation and Bioengineering vol. 84, No. 6, 519-523 1997.
Orikasa et al. Characterization of the eicosapentaenoic acid biosynthesis gene cluster fromShewanellasp. strain SCRC-2738, Cellular and Molecular Biology (Noisy-le-grand), Jul. 2004, vol. 50, No. 5, pp. 625-630.
Qiu et al. Identification of a delta4 fatty acid desaturase fromThraustochytriumsp. involved in the biosynthesis. J. Biol. Chem. Aug. 24, 2001, vol. 276, No. 34, pp. 31561-31566.
Satomi et al.Shewanelia marinintesinasp. nov.,Shewanella schlegelianasp. nov. andShewanelia sairaesp. nov., novel eicosapentaenoic-acid-producing marine bacteria isolated from see-animal intestines. Internat. J. Syst. Evol. Microbiol. 2003, vol. 53, pp. 491-499.
Singh et al. Microbial Production of Docosahexaenoic Acid (DHA, C22:6) Adv. Appl. Microbial, 1997. vol. 45, pp. 271-312.
Takeyama et al. Expression of eicosapentaenoic acid synthesis gene clustter fromShewanellasp. in transgenic marine cyanobacterium.Synechecoccussp. Microbiology. 1997, vol. 143, pp. 2725-2731.
UniProt Accession No. Q93CG6—PHOPR, (Allen et al.) 2002.
Weete et al. Lipids and Ultrasctructure ofThrauchytriumsp. ATCC26185. 1997, Am Oil Chem. Soc. vol. 32, No. 8, pp. 839-845.
Yokochi et al. Optimization of docosahexaenoic acid production. App. Microbiol. Biotechnol. 1998, vol. 49, pp. 72-76.
International Search Report for International (PCT) Patent Application No. PCT/US02/12254, mailed Nov. 15, 2002.
International Preliminary Examination Report for International (PCT) Patent Application No. PCT/US02/12254, mailed Oct. 16, 2006.
International Search Report for International (PCT) Patent Application No. PCT/US00/00956, mailed Jul. 6, 2000.
Written Opinion for International (PCT) Patent Application No. PCT/US00/00956, mailed Dec. 19, 2000.
International Preliminary Examination Report for International (PCT) Patent Application No. PCT/US00/00956, mailed Apr. 19, 2001.
International Search Report for International (PCT) Patent Application No. PCT/US04/09323, mailed Apr.

Affiliated with

Also associated with

Rating

PUFA polyketide synthase systems and uses thereof does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with PUFA polyketide synthase systems and uses thereof, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and PUFA polyketide synthase systems and uses thereof will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2678300