PSM112 plasmid vector for expression in bacillus subtilis

Details PSM112 plasmid vector for expression in bacillus subtilis PSM112 plasmid vector for expression in bacillus subtilis

1995-03-07

1996-12-17

Wax, Robert A.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435 691, 4351723, 43525235, 4353701, 935 23, 935 29, 935 74, 935 48, C12N 986, C12N 1555, C12N 1564, C12N 1574

Patent

active

055852602

ABSTRACT:
pSM112 plasmid vector used for cloning heterologous proteins in Bacillus subtilis. A method of constructing plasmid pSM112 from plasmid pSM23 which contains the origin of replication of pUB110 and of pE194, said method comprising: a) plasmid pSM23 is linearized with XbaI restriction enzyme, b) the plasmid DNA is cyclized with T4 DNA ligase enzyme and plasmid pSM29 is isolated, c) the EcoRI-BamHI fragment of pSM29 containing the replication origin of pE194 is removed, d) the large EcoRI-BamHI fragment of pSM29 containing the replication origin of pUB110 is cyclized, and e) the plasmid pSM112 is isolated.

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