Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Recombinant virus encoding one or more heterologous proteins...
Reexamination Certificate
2006-04-25
2006-04-25
Parkin, Jeffrey S. (Department: 1648)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Recombinant virus encoding one or more heterologous proteins...
C424S218100
Reexamination Certificate
active
07033595
ABSTRACT:
Cells that produce inventive pseudotyped retroviruses having a broad host range have been produced. In one aspect of the invention, the cells produce retroviruses pseudotyped with at least two different viral glycoproteins, such as togaviral glycoproteins. In alternative embodiments, the cells produce retroviruses pseudotyped with filoviral glycoproteins. Methods of producing the above-described cells, as well as the pseudotyped retroviruses thus produced, are also provided. In other embodiments, methods of screening agents effective in blocking viral entry into a cell, including filoviral entry or entry of viruses having at least two different viral glycoproteins disposed in their lipid bilayer, such as togaviruses, are provided. Moreover, methods of using the inventive pseudotyped retroviruses for introducing nucleotide sequences into target cells, and kits for forming the inventive pseudotyped retroviruses, are also provided.
REFERENCES:
patent: 4912030 (1990-03-01), Weiss et al.
patent: 5185440 (1993-02-01), Davis et al.
patent: 5278056 (1994-01-01), Bank et al.
patent: 5491084 (1996-02-01), Chalfie et al.
patent: 5503974 (1996-04-01), Gruber et al.
patent: 5512421 (1996-04-01), Burns et al.
patent: 5591624 (1997-01-01), Barber et al.
patent: 5681746 (1997-10-01), Bodner et al.
patent: 5723287 (1998-03-01), Russell et al.
patent: 5723333 (1998-03-01), Levine et al.
patent: 5739018 (1998-04-01), Miyanohara et al.
patent: 5747243 (1998-05-01), Gruber et al.
patent: 5750396 (1998-05-01), Yang et al.
patent: 5910434 (1999-06-01), Rigg et al.
Will, C., et al.,Marburg Virus Gene 4 Encodes the Virion Membrane Protein, a Type I Transmembrane Glycoprotein, J. of Virology, 67:3:1203-1210 (1993).
Sanchez, A., et al.,Sequence Analysis of the Ebola Virus Genome: Organization, Genetic Elements, and Comparison with the Genome of Marburg Virus, Virus Research, 29:215-240 (1993).
Lopez, S., et al.,Nucleocapsid-Glycoprotein Interactions Required for Assembly of Alphaviruses, J. of Virology, 68:3:1316-1323 (1994).
Riviere, I, et al.,Effects of Retroviral Vector Design on Expression of Human Adenosine Deaminase in Murine Bone Marrow Transplant Recipients Engrafted with Genetically Modified Cells, Proc. Nat'l. Acad. Sci. USA, 92:6733-6737 (1995).
Sharma, S., et al.,Efficient Infection of a Human T-Cell Line and of Human Primary Peripheral Blood Leukocytes with a Pseudotyped Retrovirus Vector, Proc. Nat'l Acad. Sci. USA, 93:21:11842-11847 (1996).
Ory, D. S., et al.,A Stable Human-Derived Packaging Cell Line for Production of High Titer Retrovirus/Vesicular Stomatitis Virus G Pseudotypes, Proc. Nat'l. Acad. Sci. USA, 93:11400-11406 (1996).
Kuhn, R. J., et al.,Chimeric Sindbis-Ross River Viruses to Study Interactions Between Alphavirus Nonstructural and Structural Regions, J. of Virology, 70:11:7900-7909 (1996).
Takada, A., et al.,A System for Functional Analysis of Ebola Virus Glycoprotein, Proc. Nat'l. Acad. Sci. USA, 94:14764-14769 (1997).
Grignani, F., et al.,High-Efficiency Gene Transfer and Selection of Human Hematopoietic Progenitor Cells with a Hybrid EBV/Retroviral Vector Expressing the Green Fluorescence Protein, Cancer Research, 58:14-19 (1998).
Yang, Z., et al.,Distinct Cellular Interactions of Secreted and Transmembrane Ebola Virus Glycoproteins, Science, 279:1034-1037 (1998).
Wool-Lewis, R. and Bates, P.,Characterization of Ebola Virus Entry by Using Pseudotyped Viruses: Identification of Receptor-Deficient Cell Lines, J. of Virology, 72:4:3155-3160 (1998).
Hatzhoannou, T., et al.,Incorporation of Fowl Plague Virus Hemagglutinin into Murine Leukemia Virus Particles and Analysis of the Infectivity of the Pseudotyped Retroviruses, J. of Virology, 72:6:5313-5317 (1998).
Blanton et al. “Plasmid transfection and retroviral transduction of porcine muscle cells for cell-mediated gene transfer.”J. Anim. Sci.2000;78(4):909-18.
Current Protocols in Molecular Biology, Ausubel et al. eds. 1988. Table of Contents.
Faragher et al. “Genome Sequences of a Mouse-Avirulent and a Mouse-Virulent Strain of Ross River Virus”Virology1988;163:509-526.
Jeffers et al. “Covalent modifications of the ebola virus glycoprotein.”J. Virol.2002;76(24):12463-72.
Kang et al. “In vivo gene transfer using a nonprimate lentiviral vector pseudotyped with Ross River Virus glycoproteins.”J Virol.2002;76(18):9378-88.
Kuhn et al. “Infectious RNA Transcripts from Ross River Virus cDNA Clones and the Construction and Characterization of Defined Chimeras with Sindbis Virus”Virology1991;182:430-441.
Lodge R, et al. “Two distinct oncornaviruses harbor an intracytoplasmic tyrosine-based basolateral targeting signal in their viral envelope glycoprotein.”J Virol.1997;71(7):5696-702.
Markowitz et al. “A safe packaging line for gene transfer: separating viral genes on two different plasmids.”J Virol.1988;62(4):1120-4.
Marsh et al. “Virus entry into animal cells.”Adv Virus Res.1989;36:107-51.
Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory 1989. Table of Contents.
Morgenstern et al. “A series of mammalian expression vectors and characterisation of their expression of a reporter gene in stably and transiently transfected cells.”Nucleic Acids Res.1990;18(4):1068.
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus EVU23187 bp RNA, Accession No. U23187, “Zaire Ebola virus Mayinga strain glycorotein (GP) gene, compete cds.” [online]. Bethesda, MD (Feb. 8, 2003).<URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=nucleotide&list—uids=10, (3 pgs.).
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus MVREPCYC 2845 bp DNA, Accession No. Z12132, “Marburg virus genes for vp35, vp40, vp30 vp24, glycoprotein, nucleoprotein, polymerase,” [online]. Bethesda, MD (Feb. 10, 2003).<URL: http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?cmd=&txt=&save=&cfm=&query—key=2&db=nucleotide&Extrafeat=-1&view+def&dispmax=20&SendTo=on&from=5822&—to8666&—Strand=, (3 pgs.).
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, GenBank Locus MVREPCYC 19104 bp DNA, Accession No. MVREPCYC, “Marburg virus genes fro vp35,vp40, vp30, vp24, glycoprotein, nucleoprotein, polymerase,” [online]. Bethesda, MD (Aug. 26, 2002).<URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=Nucleotide&list—uids=5, (11 pgs.).
Pear et al. “Production of high-titer helper-free retroviruses by transient transfection”Proc Natl Acad Sci U S A.1993;90(18):8392-6.
Prasher et al. “Primary structure of the Aequorea victoria green-fluorescent protein.”Gene.1992;111(2):229-33.
Retroviruses, Cold Spring Harbor Laboratory Press, ed. By Coffin et al. 1997;444.
Sanders DA. “No false start for novel pseudotyped vectors.”Curr Opin Biotechnol.2002;13(5):437-42.
Sanes et al. “Use of a recombinant retrovirus to study post-implantation cell lineage in mouse embryos.”EMBO J.1986;5(12):3133-42.
Smith et al. “Putative receptor binding sites on alphaviruses as visualized by cryoelectron microscopy.”Proc Natl Acad Sci U S A. 1995;92(23):10648-52.
Strauss et al. “The alphaviruses: gene expression, replication, and evolution.”Microbiol Rev.1994;58(3):491-562.
Taylor, G. M. and D. A. Sanders. 1999. The role of the membrane-spanning-domain sequence in glycoprotein-mediated membrane fusion.Mol. Biol. Cell10:2803-2815.
Taylor et al. “Fv-4: identification of the defect in Env and the mechanism of resistance to ecotropic murine leukemia virus.”J Virol.2001;75(22):11244-8.
Van Beveren et al. “Nucleotide sequence of the genome of a murine sarcoma virus.”Cell1981;27(1 Pt 2):97-108.
Fishbach Michael A.
Jeffers Scott A.
Kuhn Richard J.
North Cynthia L.
Sanders David A.
Mueting Raasch & Gebhardt, P.A.
Parkin Jeffrey S.
Purdue Research Foundation
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