Pseudorabies virus deletion mutants involving the EPO and LLT ge

Details Pseudorabies virus deletion mutants involving the EPO and LLT ge Pseudorabies virus deletion mutants involving the EPO and LLT ge

1992-09-11

1994-10-04

Nucker, Christine M.

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Virus or component thereof

4242291, 424815, 435236, 4352351, 435948, 4351723, 935 10, 935 12, A61K 39245, C12N 704

Patent

active

053525965

ABSTRACT:
An attenuated pseudorabies virus (PRV) having a reduced ability to reactivate from latency is produced by introducing (1) a genomic modification in the early protein 0 (EP0) gene whereby said virus is characterized by the inability to express the early protein 0; or (2) a genomic modification in the large latency transcript (LLT) gene whereby said virus is characterized by disruption of the synthesis of said large latency transcript; or (3) the genomic modifications described in both (1) and (2). The attenuated virus is useful in a vaccine for psuedorabies-susceptible animals, particularly swine. Swine vaccinated with a deletion mutant in the EP0/LLT overlap region displayed reduced virus shedding and fewer clinical signs than animals inoculated with a wild type virus. The deletion mutant-vaccinated swine also harbored less PRV DNA in the nervous tissue and showed reduced ability to reactivate the virus.

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