Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1990-03-30
1993-05-11
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435874, 436518, G01N 33569
Patent
active
052100197
ABSTRACT:
A screening assay for detecting the presence of Pseudomonas bacteria in a test sample comprising isolating the bacteria present in the sample, extracting Azurin protein from the periplasm within the bacteria, reacting the extract with an enzymatically labelled, Azurin-specific antibody and a color-producing substrate specific for the enzyme which is capable of producing a color distinct from the color generated by Azurin and reading the results of the reaction of the sample with the antibody to determine whether the color has been produced whereby the presence of Pseudomonas bacteria in the sample is indicated.
REFERENCES:
patent: 4963480 (1990-10-01), Belcour et al.
Stahey et al., J. Bacteriol., vol. 43, No. 2, pp. 149-154, (1942).
Trees et al., J. Inf. Dis., vol. 161, pp. 336-339. (1990).
A. B. Champion, K. L. Soderbere, & A. C. Wilson, "Immunological Comparison of Azurins of Known Amino Acid Sequence," J. Mol. Evol. 5, 291-305 (1975).
Nenad M. Kostic, Ruth Margalit, Chi-Ming Che & Harry B. Gray, "Kinetics of Long-Distance Ruthenium-to-Copper Electron Transfer in (Pentaammineruthenium histidine-83) Azurin", Journal of the American Chemical Society, 1983, 105, 7765.
D. Y. Mason, M. Naiem, Z. Abdulaziz, J. R. G. Nash, K. C. Gatter & H. Stein, Immuno histological Applications of Monoclonal Antibodies, pp. 585-613.
Joseph S. Lam, Lucy M. Mutharia & Robert E. W. Hancock, "Application of Monoclonal Antibodies to the Study of te Surface of Antigens in Pseudomonas aeruginosa, Monoclonal Antibodies Against Bacteria," vol. II, 143-157.
Jones Thomas H.
Kepplinger Esther L.
Kusmiss John H.
Miller Guy M.
The United States of America as represented by the Administrator
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