Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1992-07-31
1996-07-09
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4353201, 536 232, C12N 920, C12N 1555, C12N 1570
Patent
active
055344275
ABSTRACT:
Nucleic acid sequences, particularly DNA sequences, coding for all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the BamHI/HindIII insert of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 deposited with the American Type Culture Collection, expression vectors containing the DNA sequences, host cells containing the expression vectors, and methods utilizing these materials. The invention also concerns polypeptide molecules comprising all or part of a lipase of Pseudomonas fluorescens, wherein said lipase has the amino acid sequence encoded by the nucleotide sequence of the plasmid pRJ-Ltac1 from Escherichia coli BL21/pRJ-Ltac1 BamHI/HindIII insert of deposited with the American Type Culture Collection, and methods for producing these polypeptides.
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Cino Paul M.
Goldberg Steven L.
Johnston Robert M.
E. R. Squibb & Sons Inc.
Gaul Timothy J.
Patterson Jr. Charles L.
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