Pseudomonas degradation of hydrocarbons

Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses

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435 91, 435253, 435317, 435874, 935 29, 935 52, 935 59, 935 72, C12N 1500, C12N 120, C12N 100, C12P 1934, C12R 138

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045088241

ABSTRACT:
Selected new bacteria of the genus Pseudomonas, particularly the species Pseudomonas putida and Pseudomonas aeruginosa, which have the ability to utilize organic compounds from the generic groups aliphatic, cyclo aliphatic, aromatic and/or polynuclear aromatic hydrocarbons are described. The source of genetic materials facilitating degradation of the aromatic compounds are metabolic plasmids. In particular, Pseudomonas putida or other Pseudomonas obtained from soil samples and having a non-transmissible and stable ability to degrade hexane (as well as related aliphatic hydrocarbons) are used as starting strains to produce the new bacteria. Transconjugal mating and selection for these genetic traits resulted in the production of bacteria capable of utilizing representative compounds of all the generic groups of the previously listed organic compounds. The bacteria are useful for waste degradation.

REFERENCES:
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patent: 4259444 (1981-03-01), Chakrabarty
patent: 4374200 (1983-02-01), Olsen
Novick, Bact. Rev. 33:210-235(1969).
Jacoby et al, Nature, 274:179-180(1978).
Holloway, B. W., Bacteriological Reviews, 33, 419-443 (1969).
Vandenbergh et al, Appl. and Environ. Microbiol., 42:737-739, 1981.
Olsen et al, J. Bacteriol 123:28-35 (1975).
Atlas, R. H., Microbiol. Rev. 45:180-209 (1981).
Olsen, J. Bacteriol. 133:210-216(1978).

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