Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1991-06-18
1993-11-09
Saunders, David
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 5, 435 723, 435 724, 435 732, 435 29, 435 30, 435243, 436 63, 436 64, 436503, 436805, 436811, 436813, 436827, C12Q 102, C12Q 124, G01N 33567, G01N 33569
Patent
active
052601862
ABSTRACT:
An improved method is provided for the isolation of lymphocytes for use in the SCM test for the detection of cancer and other diseases and conditions. The method of the present invention can isolate either or both of the SCM-responding lymphocyte fractions from the same blood sample: the F2 fraction, with a buoyant density of 1.0590 g/cm.sup.3 to 1.0670 g/cm.sup.3 measured at 20.degree. C. in a solution having an osmolality of about 0.315 Osm/kg, and the F4 fraction, with a buoyant density of 1.0690 g/cm.sup.3 to 1.0730 g/cm.sup.3. These lymphocyte fractions are isolated in visible bands and are substantially free of lymphocytes having other buoyant densities. This avoids cross-contamination of the lymphocyte fractions with each other as well as with SCM non-responding lymphocytes. Several gradients useful in this isolation method are described. This isolation method can use as starting material either a blood sample depleted of phagocytic cells or the total population of lymphocytes. The latter avoids the use of iron powder or carbonyl-iron powder in removing the phagocytic cells.
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Cercek Boris
Cercek Lea
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