Proteted chemiluminescent labels

Details Proteted chemiluminescent labels Proteted chemiluminescent labels

1988-02-26

1990-08-21

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving producing or treating antigen or hapten

Producing labeled antigens

436544, 436501, 546102, 546103, 546104, 546110, 546169, 546170, G01N 33533, G01N 33532, C07D26538, C07D29500

Patent

active

049506137

ABSTRACT:
A method of preparing a labelled specific binding partner, such as a biological probe in the form of an antibody or oligonucleotide probe, using a protected label (the corresponding unprotected label being susceptible to inactivation, such as by hydrolysis to yield a non-chemiluminescent form of the label). The specific binding partner is linked to the label, and an adduct of the label is prepared using a protective adduct former which produces a protected label which is less susceptible to inactivation. Particularly preferred labels are the acridiniums and acridans, most preferably the former having the general structure: ##STR1## wherein the phenyl rings are optionally additionally substituted, R.sub.1 is preferably an alkyl, and R.sub.5 is an optionally substituted hydrocarbon, most preferably a phenyl moiety which is either linked to the specific binding partner, or capable of being linked thereto. Formation of the protected label is preferably an equilibrium reaction which is readily reversible, such as by dilution or oxidation of the protective adduct former. Labels useful in such a method are also disclosed.

REFERENCES:
patent: 4372745 (1983-02-01), Mandle et al.
patent: 4745181 (1988-05-01), Law et al.
McCapra, "Proposal in Organic Chemistry", vol. 8, pp. 231-277 (1973).
Weeks, et al., Clin. Chem., 29/8, pp. 1474-1479 (1983).
McCapra, "Accounts of Chemical Research", vol. 9, No. 6, pp. 201-208 (Jun. 1976).
Colowick et al., J. Biol. Chem., vol. 191, p. 447 (1951).
Ludwieg, et al. Biochem. Biophys. Res. Commun. vol. 14, p. 431 (1964).
"Acridines", Publs. John Wiley & Sons, Inc., London, U.K. (1973, ed., R. M. Acheson).

Affiliated with

Also associated with

Rating

Proteted chemiluminescent labels does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Proteted chemiluminescent labels, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Proteted chemiluminescent labels will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1677319