Proteins with mutations to decrease N-terminal methylation

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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4351721, 4352523, 4353201, 530350, 530385, C12P 2106, C12N 120, C12N 1500, C07K 100

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061400718

ABSTRACT:
This invention relates to proteins with N-terminal modifications in their amino acid sequence that reduce or eliminate the methylation of the N-terminal methionine that occurs when such proteins are produced in bacteria such as E. coli.

REFERENCES:
patent: 4377512 (1983-03-01), Ajisaka et al.
patent: 4600531 (1986-07-01), Walder
patent: 4696816 (1987-09-01), Brown
patent: 4831012 (1989-05-01), Estep
patent: 4861867 (1989-08-01), Estep
patent: 4873192 (1989-10-01), Kunkel
patent: 5084558 (1992-01-01), Rausch et al.
Brittis et al., "Chondroitin Sulfate as a Regulator of Neuronal Patterning in the Retina" Science, vol. 255, pp. 733-736, (Feb. 7, 1992).
Caterson et al., "Modulation of native chondroitin sulphate structure in tissue development and in disease" Journal of Cell Science, vol. 97, Part 3, pp. 411-417, (Nov. 1990).
Guthrie and Salyers, "Evidence that the Bacteroides thetaiotaomicron Chondroitin Lyase II Gene Is Adjacent to the Chondro-4-Sulfatase Gene and May Be Part of the Same Operon" Journal of Bacteriology, vol. 169, No. 3, pp. 1192-1199, (Mar., 1987).
Henderson et al., "Nucleolysis of the Rabbit Intervertebral Disc Using Chondroitinase ABC" Spine, vol. 16, No. 2, pp. 203-208, (1991).
Kato et al., "Experimental Chemonucleolysis With Chondroitinase ABC" Clinical Orthopaedics and Related Research , vol. 253, pp. 301-308, (Apr., 1990).
Linker et al., "The Formation of Unsaturated Disaccharides from Mucopolysaccharides and Their Cleavage to .alpha.-Keto Acid by Bacterial Enzymes" Journal of Biological Chemistry, vol. 235, No. 11, pp. 3061-3065, (Nov., 1960).
McKeon et al., "Reduction of Neurite Outgrowth in a Model of Glial Scarring following CNS Injury Is Correlated with the Expression of Inhibitory Molecules on Reactive Astrocytes" The Journal of Neuroscience, vol. 11, No. 11, pp. 3398-3411, (1991).
Neuberg and Hofman, "Enzymatischer Abbau von Chondroitinschwefelsaure" Biochemische Zeitschrift, vol. 234, pp. 345-346, (1931). (No translation).
Neuberg and Rubin, "Uber die Bildung von Thioschwefelsaure und Schwefelsaure aus Atherschwefelsauren und Sulfonsauren" Biochemische Zeitschrift, vol. 67, pp. 82-89, (1914). (No translation).
Pettipher et al., "Cartilage Proteoglycan Depletion in Acute and Chronic Antigen-Induced Arthritis" Arthritis and Rheumatism, vol. 32, No. 5, pp. 601-607, (May, 1989).
Reissig et al., "A Modified Colorimetric Method for the Estimation of N-Acetylamino Sugars" The Journal of Biological Chemistry, vol. 217, pp. 959-966, (1955).
Saito et al., "Enzymatic Methods for the Determination of Small Quantities of Isomeric Chondroitin Sulfates" The Journal of Biological Chemistry, vol. 243, No. 7, pp. 1536-1542, (Apr. 10, 1968).
Sato et al., "Cultural Conditions for the Induction of Chondroitinase ABC in Proteus vulgaris Cells" J. Ferment. Technol., vol. 64, No. 2, pp. 155-159, (1986).
Sato et al., "Growth-Promoting Activity of Tuna Growth Hormone and Expression of Tuna Growth Hormone cDNA in Escherichia coli" Biotechnology and Applied Biochemistry, vol. 10, pp. 385-393, (1988).
Sato et al., "Subunit Structure of Chondroitinase ABC from Proteus vulgaris" Agric. Biol. Chem., vol. 50, No. 4, pp. 1057-1059, (1986).
Seibel et al., "Antigen Properties of Keratan Sulfate: Influence of Antigen Structure, Monoclonal Antibodies, and Antibody Valency" Archives of Biochemistry and Biophysics, vol. 296, No. 2, pp. 410-418, (Aug. 1, 1992).
Thurston, "Induction and Catabolite Repression of Chondroitinase in Batch and Chemostat Cultures of Proteus vulagris" Journal of General Microbiology, vol. 80, pp. 515-522, (1974).
Yamagata et al., "Purification and Properties of Bacterial Chondroitinases and Chondrosulfatases" The Journal of Biological Chemistry, vol. 243, No. 7, pp. 1523-1535, (Apr. 10, 1968).
Brown et al., Intradiscal Therapy, Year Book Medical Publishers, inc. Chicago, (1983).
Sato et al., Biotechnol. Bioeng., vol. 28, pp. 1707-1712, (1986).
De Boer, H.A. et al/The Tac Promoter: A Functional Hybrid Derived from the TRP and LAC Promoters/PNAS/(1983), 80: 21-25.
Dente/DNA Cloning/Glover Ed./IRL Press/(1985), 101-107.
Dickerson, R.E. & Geis, I./Hemoglobin, Structure, Function, Growth and Pathology/ Evolution of the Oxygen Carriers/(1983), Chapter 3/66-115/Benjamin Cummings Publishing Co/California.
Didonato, A. et al/Selective Carboxymethylation of the .alpha.-Amino Groups of Hemoglobin Effect on Functional Properties/J. Biological Chemistry/(1983), 258(19): 11890-11895.
Farabaugh, P.J./Sequence of the lacI Gene/Nature/(1978), 274: 765-769.
Fujiyama, A & Tamanoi, F./RAS2 Protein of Saccharomyces Cerevisiae Undergoes Removal of Methionine at N Terminus and Removal of Three Amino Acids at C Terminus/(1990), 265: 3362-3368.
Hirel, H. et al/Extent of N-Terminal Methionine Excision From Escherichia Coli Proteins is Governed by the Side-Chain Length of the Penultimate Amino Acid/PNAS/(1989), 86: 8247-8251.
Jones, D.H. & Howard, B.H./A Rapid Method for Site-Specific Mutagenesis and Directional Subcloning by Using the Polymerase Chain Reaction to Generate Recombinant Circles/Biotechniques/(1990), 8(2): 178-183.
Jones, D.H. & Howard, B.H./A Rapid Method for Recombination and Site-Specific Mutagesesis by Placing Homologous Ends on DNA Using Polymerase Chain Reaction/Biotechniques/ (1991), 10(1), 62-66.
Kunkel, T.A. et al./Rapid and Efficient Site-Specific Mutagenesis Without Phenotypic Selection/Methods in Enzymololgy/(1987), 154: 367-382.
Looker, D. et al/A Human Recombinant Haemoglobin Designed for Use as a Blood Substitute/Nature/(1992), 356: 258-260.
Luisi, B.F. & Nagai, K./Crystallographic Analysis of Mutant Human Haemoglobins Made in Escherichia Coli/Nature/(1986) 320: 555-556.
McCracken, A.A. et al/An Enrichment Selection for Mutants Resulting from Oligo-Nucleotide-Directed Mutagenesis of Double-Stranded DNA/Biotechniques/(1988) 6(4): 332-339.
Morgan, D.G. et al/Proteins Antigenically Related to Methyl-Accepting Chemotaxis Proteins of Escherichia Coli Detected in a Wide Range of Bacterial Species/J. Bacteriology/(1993), 175(1): 133-140.
Nagai, K. et al/Distal Residues in the Oxygen Binding Site of Haemoglobin Studied by Protein Engineering/Nature/(1987) 329: 858-860.
Zoller, M.J. & Smith, M./Oligonucleotide-Directed Mutagenesis of DNA Fragments Cloned into M13 Vectors/Methods in Enzymology/(1983) 100: 468-500.
Nakamaye, K.L. & Eckstein, F./Inhibition of Restriction Endonuclease NCII Cleavage by Phosphorothioate Groups and its Application to Oligonucleotide-Directed Mutagenesis/Nucleic Acids Research/(1986) 14(24): 9679-9698.
Paik, W.K./A Simple Analysis of Methylated Proteins/Methods in Enzymology/(1984) 106: 265-268.
Pasloske, B.L. & Paranchych, W./The Expression of Mutant Pilins in Pseudomonas Aeruginosa: Fifth Position Glutamate Affects Pilin Methylation/Molecular Microbiology/(1988) 2(4): 489-495.
Schulz, G.E. & Shirmer, R.H./Principles of Protein Structure/(1979) Table 1-2/Springer-Verlag.
Stock, A./N-Methylmethionine at the Amino Terminus of a Protein Required for Bacterial Chemotaxis/Advances in Post-Translational Modification of Proteins & Aging/Ed. Zappia/(1987) 387-399.
Stock, A. et al/N-Terminal Methylation of Proteins: Structure, Function and Specificity/ FEBS Letters/(1987) 220(1): 8-14.
Stock, A. et al/A Second Type of Protein Methylation Reaction in Bacterial Chemotaxis/ J. of Biological Chemistry/(1987) 262(17): 8011-8014.
Stock A.M. & Stock, J.B./Purification and Characterization of the Chez Protein of Bacterial Chemotaxis/J. of Bacteriology/(1987) 169(7): 3301-3311.
Strom, M.S. & Lory, S./Amino Acid Substitutions in Pilin of Pseudomonas Aeruginosa Effect on Leader Peptide Cleavage, Amino-Terminal Methylation, and Pilus Asssembly/J. of Biological Chemistry/(1991) 266(3): 1656-1664.
Sutcliffe, J.G./ Complete Nucleotide Sequence of the Escherichia Coli Plasmid PBR322/Cold Spring Harbor Symp. Quant. Biol.(1979) 43: 77-90.
Taylor, J.W. et al/The Rapid Generation of Oligonucleotide-Directed Mutations at High Frequency Using Phosphorothioate-Modified DNA/Nucleic Acids Research/(1985) 13(24): 8765-8785.
Taylor, J.W. et al/The Use of Phosphorothioate-Modifi

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