Proteins, their production and use

Details Proteins, their production and use Proteins, their production and use

1997-04-04

1999-02-23

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

435226, 435 76, 435 79, 435 691, 530350, C12N 950

Patent

active

058742778

ABSTRACT:
This invention relates to a novel calpain having a proteolytic activity, its partial peptide or a salt either of them, a DNA coding for the protein, a recombinant vector comprising the DNA, a transformant carrying the recombinant vector, a process for producing the protein, a pharmaceutical composition comprising the DNA, an antibody against the protein, a method for screening for a compound which activates or inhibits a proteolytic activity of the protein, a kit for screening for the compound, and a compound which activates or inhibits a proteolytic activity of the protein which is identified by the screening method or the kit. The DNA coding for the protein of the present invention can be used as a therapeutic and prophylactic composition for a variety of diseases including tumor, cerebral apoplexy, cerebral infarction, subarachnoid hemorrhage, Alzheimer's disease, myodystrophy, cataract, ischemic heart disease, atherosclerosis, arthritis, and collagen disease. Furthermore, the protein of the present invention is useful as a screening reagent for any compounds which activates or inhibits the function of the protein of the present invention. In addition, the antibody against the protein of the present invention specifically recognizes the protein of the present invention and can be used in the quantitative determination of the protein of the present invention in a test fluid.

REFERENCES:
Legendre, et al., Inflammation. vol. 12, No. 1, pp. 51-65 (1988).
Saido, et al., The FASEB Journal, vol. 8, pp. 814-822 (1994).
Sorimachi, et al., J. of Biol. Chem., vol. 268, No. 26, pp. 19476-19482 (1993).
Menard, et al., Immunology Today vol. 17, pp. 545-547 (1996).
Legendre et al. (1988) Inflammation 12:51-65, 1988.
Sorimachi et al. (1993) J. Biol. Chem. 268/26:19476-19482, 1993.
Sorimachi et al. (1997) GenBank Database, Accession No. A48764, 1997.
Takano et al. (1988) Biochemistry International 16/3:391-395, 1988.
Saito, K-I et al. Widespread activation of calcium-activated neutral proteinase (calpain) in the brain in Alzheimer disease: A potential molecular basis for neuronal degeneration. Neurobiology, vol. 90, 1993, 2628-2632.
Sorimachi, H. et al. A Noveltissue-specific calpain species expressed predominantly in the stomach comprises two alternative splicing products with and without Ca.sup.2+ -binding Domain*. The Journal of Biological Chemistry, vol. 90, 1993, 19476-19482.

Affiliated with

Also associated with

Rating

Proteins, their production and use does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Proteins, their production and use, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Proteins, their production and use will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-305935