Proteins having glucose transporter activity

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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C514S012200

Reexamination Certificate

active

10451822

ABSTRACT:
The present invention provides proteins having the Na+/glucose transporter activity, DNAs encoding the proteins, a method of screening for a compound enhancing or inhibiting the activity of the proteins, and compounds obtained by the screening method. The proteins having the same or substantially the same amino acid sequence as the amino acid sequence represented by SEQ ID NO: 1, 15 or 26 are useful as a diagnostic marker for a disease such as diabetes. The compounds enhancing or inhibiting the activity of the proteins, obtained the screening method using the proteins, can be used as a prophylactic and/or therapeutic agent for a disease such as diabetes and hyperlipidemia.

REFERENCES:
patent: 2005/0196754 (2005-09-01), Drmanac et al.
patent: WO 00/18918 (2000-04-01), None
Database EMBL: Nov. 24, 2000; XP002305383; Database accession No. AL 109659/ the N-terminal part of the encoded protein of accession No. Q9NPZ7 (TrEMBL) is 100% identical to SEQ ID. No. 1 in a 68 amino acids overlap.
David G. Wang, et al., “Large-Scale Identification, Mapping, and Genotyping of Single-Nucleotide Polymorphisms in the Human Genome”, Science, (1998), pp. 1077-1082, vol. 280.
Jason T. Lam, et al., “Missense Mutations in SGLT1 Cause Glucose-Galactose Malabsorption by Trafficking Defects”, Biochimica et Biophysia Acta, (1999), pp. 297-303, vol. 1453, No. 2.
Kenji Tsujihara, “Na+-Glucose Cotransporter (SGLT) Inhibitors as Antidiabetic Agents. 4. Synthesis and Pharmacological Properties of 4'-Dehydroxyphlorizin Derivatives Substituted on the B Ring”, Journal of Medicinal Chemistry, (1999), pp. 5311-5324, vol. 42, No. 26.
Tarpey, et al. “Amino acid sequence and the cellular location of the Na+-dependent D-glucose symporters (SGLT1) inthe ovine enterocyte and the parotid acinar cell” BIOCHEM J 312:293-300 (1995).
Ana M. Pajor, “Sequence of aputative transporter from rabbit kidney related to the Na+/glucose cotransporter gene family” Biochem. Biophys. Acta 1194(2): 349-351 (1994).
Turk, et al. Structure of the Human Na+/Glucose Cotransporter Gene SGLT1 J. Biol. Chem. 269(21): 15204-15209 (1994).
Yang, et al. “Expression characteristics and relevance of sodium glucose cotransporter-1 in mammalian renal tubulogenesis” Am. J. Physiol. Renal Physiol. 279(4): F765-777 (2000).
Oku, et al. “T-1095, an Inhibitor of Renal Na+-Glucose Cotransporters, May Provide a Novel Approach to Treating Diabetes” DIABETES 48: 1794-1800 (1999).
C.I. Cheeseman, “Upregulation of SGLT-1 Transport Activity in Rat Jejunum Induced by GLP-2 Infusion in vivo” Am. J. Physiol. 273: R1965-1971 (1997).
Nippon Rinsho (Japan Clinical), 55: 59-64 (1997), Extra Volume “Diabetes 1”.

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