Proteins from yeast host strains with defects in N-glycosylation

Details Proteins from yeast host strains with defects in N-glycosylation Proteins from yeast host strains with defects in N-glycosylation

1995-06-05

1998-01-06

Wax, Robert A.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Glycoprotein, e.g., mucins, proteoglycans, etc.

43525411, 43525421, C07K 100, C12N 114, C12N 116, C12N 118

Patent

active

057056161

ABSTRACT:
Saccharomyces mutants with defects in N-glycosylation which are obtainable selective markers, selection of those strains which, after transformation with the plasmid YEpL/glucose oxidase, secrete 10 mg/l GOD or more into the culture medium after culture under standard conditions, are allelic to the ngd mutations in Saccharomyces cerevisiae, DSM 7042, DSM 7338, DSM 7160 and/or 7340 and express proteins with a uniform carbohydrate structure.

REFERENCES:
patent: 5135854 (1992-08-01), MacKay et al.
patent: 5602018 (1997-02-01), Kopetzki et al.
Nagasu et al., "Isolation of New Temperature-Sensitive Mutants of Saccharomyces cerevisiae Deficient in Mannose Outer Chain Elongation", Yeast, vol. 8: 535-547 (1992).
Nakayama et al., "OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongatioin of asparagine-linked oligosaccharides", EMBO Journal, vol. 11, No. 7, pp. 2511-2519, (1992).
Kopetzki et al. "Enzymes in Diagnostics:Achievements and Possibilities of Recombinant DNA Technology", Clin. Chem 40/5: 688-704, (1994).
Lehle et al., "Glycoprotein Biosynthesis in Yeast: Ngd29, a Yeast mutant Allelic to Och1 Having a Defect in Initiation of Outer Chain Glycosylation" (Not Published).
European Search Report dated Jan. 25, 1995.
Huffaker et al. "Temperature Sensitive Yeast Mutants Deficient in Asparagine-linked Glycosylation, The Journal of Biological Chem.", vol. 257, No. 6, Mar. 25, pp. 3203-3210, 1982.
Hirschberg et al., "Selection of Mutant Chinese Hamster Ovary Cells Altered in Glycoproteins by Means of Tritiated Fucose Suicide", Molecular and Cellular Biology, Oct. 1981, pp. 02-909.

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