Proteins bound to a marker or solid phase support matrix using a

Details Proteins bound to a marker or solid phase support matrix using a Proteins bound to a marker or solid phase support matrix using a

1987-02-09

1989-10-17

Kight, John

Synthetic resins or natural rubbers -- part of the class 520 ser

Synthetic resins

Mixing of two or more solid polymers; mixing of solid...

530395, 530810, 530811, 530812, 530813, 530814, 530815, 530816, C07G 700, C08L 8900

Patent

active

048748137

ABSTRACT:
A biological composition comprises a solid phase support matrix or marker molecule bound to protein, typically a glycoprotein such as an immunoglobulin through a linking group, spacer arm, and hydrazone group. The linking group is conveniently an ether linkage, while the spacer arm is a linear chain including six atoms, where at least one of the atoms is a tertiary amine which is protonated at a pH below about 8. The hydrazone is formed by reacting the solid phase or marker molecule having the linking arm and the spacer arm including a terminal hydrazide group with a glycoprotein oxidized to include a terminal aldehyde group. Immunological reagents prepared by linking an immunoglobulin (IgG) through a carbohydrate on the Fc or hinge regions have been found to provide very high binding capacity approaching the theoretical bivalent limit of two moles of bound antigen for every mole of bound immunoglobulin.

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patent: 4217338 (1980-08-01), Quash
patent: 4671958 (1982-03-01), Rodwell et al.
Fan and Karush (1983) Fed. Proc. 42:930 (Abstract).
Ito et al. (1986) J. Biochem. 99:1267-1272.
Quash et al. (1978) J. Immunol. Meth. 22:165-174.
Chua et al. (1984) Biochem. Biophys. Acta 800:291-300.
O'Shannessy et al. (1984) Immunol. Lett. 8:273-277
O'Shannessy et al. (1985) J. App. Biochem. 7:347-355.
Bio-Rad Bulletin 1085 (1986) "Activated Affinity Supports: Affi-Gel 10 and 15".

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