Proteinase inhibitor, precursor thereof and genetic...

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase

Reexamination Certificate

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C435S019000, C435S252300, C435S320100, C536S023200

Reexamination Certificate

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06946278

ABSTRACT:
The present invention relates generally to proteinase inhibitors, a precursor thereof and to genetic sequences encoding same. More particularly, the present invention relates to a nucleic acid molecule comprising a sequence of nucleotides which encodes or is complementary to a sequence which encodes a type II serine proteinase inhibitor (PI) precursor from a plant wherein said precursor comprises at least three PI monomers and wherein at least one of said monomers has a chymotrypsin specific site and at least one other of said monomers has a trypsin specific site.

REFERENCES:
patent: 6451573 (2002-09-01), Anderson et al.
Bryant, et al. (1976) “Proteinase Inhibitor II from Potatoes: Isolation and Characterization of its Protomer Components”:Biochemistry 15(16): 3418-3423.
Choi, et al (1990) “Primary structure of two Proteinase Inhibitor genes closely linked in the Potato genome”:Hanguk Saenghwahakho Chi 23: 214-220.
Suggs, et al. (1981) “Use of synthetic oligonucleotides as hybridization probes: Isolation of cloned cDNA sequences for human β2-microglobulin”:Proc. Nat'l. Acad. Sci. USA 78(11): 6613-6617.
Richardson (1979), Sequence Search Comparison, Result 14,FEBS Lett. 104: 322-326.

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