Protein tyrosine phosphatase substrate-trapping double...

Details Protein tyrosine phosphatase substrate-trapping double... Protein tyrosine phosphatase substrate-trapping double...

2003-01-10

2011-12-06

Swope, Sheridan (Department: 1652)

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

C435S820000, C530S389100, C530S388260

Reexamination Certificate

active

08071348

ABSTRACT:
The present invention provides protein tyrosine phosphatases (PTP) in which the invariant aspartate residue and the invariant glutamine residue are each replaced with a replacement amino acid residue, wherein the replacement residues together cause a reduction in catalytic rate (kcat) of the enzyme and an increase in substrate-binding affinity (Kd) of the enzyme. The present invention further provides methods for identifying a substrate of a PTP. Also provided are kits for identifying a substrate of a PTP. Additionally, the present invention provides methods for identifying an agent that alters interaction between a PTP and a substrate of the PTP. The invention also provides methods for reducing the activity of a substrate of a PTP.

REFERENCES:
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