Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1979-02-28
1987-05-26
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435 6, 435317, 435810, 935 20, 935 21, 935 76, C12P 2100, C12Q 168, C12N 100
Patent
active
046686240
ABSTRACT:
An improved method for in vitro synthesis of protein by in vivo processes in an extract from a living organism and containing endogenous ribosomes and endogenous transfer RNA, primarily from endogenous amino acids in said extract primarily by the use of a predetermined exogenous messenger ribonucleic acid (mRNA).
An improved method of assaying such a synthesis by the use of a labelled amino acid, which is present during said synthesis and is incorporated into said protein.
A kit for carrying out such synthesis and assay comprising a vial of substantially prokaryotic and eukaryotic cell-free extract from a living organism containing endogenous ribosomes, endogenous transfer RNA and endogenous amino acids, a vial containing translation cocktail for promoting the synthesis, and a vial containing control messenger RNA.
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patent: 3899298 (1975-08-01), Szczesniak
Pelham et al., Eur. J. Biochem. 67, 247-256 (1976).
Skup et al., Nucleic Acids Research, vol. 4, No. 10, pp. 3581-3587 (Oct. 1977).
Villa-Komaroff et al., Methods in Enzymology, vol. XXX, pp. 709-746.
Lodish, Ann. Rev. Biochem. 45, 39-72 (1976).
McDowell et al., Proc. Nat. Acad. Sci. U.S.A., vol. 69, No. 9, pp. 2649-2653 (Sep. 1972).
Sampson et al., Biochemistry, vol. 11, No. 19, pp. 3636-3640 (1972).
London et al., Federation Proceedings, vol. 35, No. 11, pp. 2218-2222 (Sep. 1976).
E.I. Du Pont de Nemours and Company
Tanenholtz Alvin E.
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