Protein synthesis

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using a micro-organism to make a protein or polypeptide

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435 68, 435172, 435317, C12P 2104, C12P 2100, C12N 1500, C12N 100

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044119941

ABSTRACT:
A plasmid or phage gene for a periplasmic or extracellular bacterial protein is cleaved, a double-stranded DNA sequence coding for a selected protein or portion thereof from a eukaryotic cell such as insulin is inserted in that cleaved gene by recombinant DNA techniques and used to transform a bacterium, and the excreted selected protein is collected.

REFERENCES:
patent: 4082613 (1978-04-01), Thirumalachar et al.
Backman et al., Proc. Nalt. Acad. Sci. U.S.A. vol. 73, No. 11, pp. 4174-4178 Nov. 1978.
Itakura et al., Science vol. 198 pp. 1056-1063 Dec. 9, 1977.
Ullrich et al., Science vol. 196 pp. 1313-1319 Jun. 17, 1977.
Scheller et al., Science vol. 196 pp. 177-180 Apr. 8, 1977.

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