Protein size marker ladder

Details Protein size marker ladder Protein size marker ladder

1993-12-02

1995-09-12

Furman, Keith C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 681, 435 691, 435 697, 435810, C07K 1400, C12P 2100, C12P 2102, C12P 2106

Patent

active

054497582

ABSTRACT:
The invention relates to a multidomain protein comprising sites for cleavage of the protein into at least 3 polypeptide domains; nucleic acid encoding the multidomain protein; a protein ladder comprising a collection of protein fragments obtained by the partial cleavage of one or more multidomain proteins by one or more cleaving agents; and methods of using and preparing the protein ladder.

REFERENCES:
patent: 4403036 (1983-09-01), Hartley et al.
patent: 4569794 (1986-02-01), Smith et al.
patent: 5030566 (1991-07-01), Son et al.
patent: 5284762 (1994-02-01), Hayano et al.
Anderson et al., J. Biol. Chem. 254:939-944 (1979).
Nefsky et al., Proc. Natl. Acad. Sci. USA 86:3549-3553 (1989).
Mazzorana et al., J. Biol. Chem. 268: 3029-3032 (1993).
Gearing, D. P. et al., Production of Leukemia Inhibitory Factor in Escherichia coli by a Novel Procedure and its Use In Maintaining Embryonic Stem Cells in Culture, Bio/Technology 7:1157-1161 (Nov. 1989).
Germino, J. et al., Rapid purification of a cloned gene product by genetic fusion and site-specific proteolysis, Proc. Natl;. Acad, Sci. USA 81:4692-4696 (Aug. 1984).
Giedroc, D. P. et al., Overexpression, Purification, and Characterization of Recombinant T4 Gene 32 Protein.sub.22-301 (G32P-B), J. Biol. Chem. 265 (20):11444-11455 (Jul. 15, 1990).
Gross, E., The Cyanogen Bromide Reaction, Methods in Enzymology 11:238-255 (1966).
Haffey, M. L. et al., Site-Specific Cleavage of a Fusion Protein by Renin, DNA 6(6):565-571 (1987).
Hartley, J. L. et al., Cloning multiple copies of a DNA segment, Gene 13:347-353 (1981).
Itakura, K. et al., Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin, Science 198:1056-1063 (Dec. 9, 1977).
Life Technologies, 1993-1994 Catalogue and Reference Guide, pp. 8-16-8-20.
Nagai, K. et al., Synthesis and Sequence-Specific Proteolysis of Hybrid Proteins Produced in Escherichia coli, Methods in Enzymology 153:461-481 (1987).
Pharmacia Biotech Inc., Molecular and Cell Biology Catalog 1993, p. 78.
Shen, S., Multiple joined genes prevent product degradation in Escherichia coli, Proc. Natl. Acad. Sci. USA 81:4627-4631 (Aug. 1984).
Szoka, P. R. et al., A General Method for Retrieving the Components of a Genetically Engineered Fusion Protein, DNA 5(1):11-20 (1986).
Villa, S. et al., Expression in Escherichia coli and Characterization of human growth-hormone-releasing factor, Eur. J. Biochem. 171:137-141 (1988).

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