Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-09-12
2009-02-17
Ketter, James S (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S254210, C435S254300, C435S254500, C435S254700, C435S254800, C435S254900
Reexamination Certificate
active
07491510
ABSTRACT:
The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.
REFERENCES:
patent: WO 02/00856 (2002-01-01), None
patent: WO 2004/081201 (2004-09-01), None
Greber et al., Effect of the glucosidase inhibitor 1-deoxynojirimycin on protein secretion fromSaccharomyces cerevisiae, Enzyme Microb. Technol., Apr. 1988, pp. 246-251, vol. 10.
Maras et al., Filamentous fungi as production organisms for glycoproteins of biomedical interest, Glycoconjugate Journal, 1999, pp. 99-107, vol. 16.
Maras et al., Structural characterization of N-linked oligosaccharides from cellobiohydrolase I secreted by the filamentous fungusTrichoderma reeseiRUTC 30, Eur. J. Biochem., 1997, pp. 617-625, vol. 245.
Simons et al., Cell wall 1,6-B-glucan synthesis inSaccharomyces cerevisiaedepends on ER glucosidases I and II, and the molecular chaperone BiP/Kar2p, The EMBO Journal, 1998, pp. 396-405, vol. 17, No. 2.
Uren et al., Abstract, Identification of paracaspases and metacaspases: two ancient families of caspase-like proteins, one of which plays a key role in MALT lymphoma, Mol Cell., Oct. 2000, pp. 961-967, vol. 6, No. 4, http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abst...(retrieved Aug. 31, 2004).
PCT International Search Report, PCT/EP2004/050277, dated Aug. 11, 2004.
Contreras Roland
Geysens Steven
Ketter James S
TraskBritt
Universiteit Gent
Vlaams Interuniversitair Institute voor Biotechnologie vzw
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