Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-07-19
1998-07-07
Degen, Nancy
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
43525421, 435320, 435325, 536 234, C12Q 168, C12N 1581, C12N 1563, C12N 2104
Patent
active
057766894
ABSTRACT:
The present invention provides a protein recruitment system, in which a protein-protein interaction is detected by the recruitment of an effector protein to a specific cell compartment, where the effector protein can activate a reporter molecule, provided that the effector protein is not a transcription factor. The invention also provides a drug screening assay using the protein recruitment system. In addition, the invention provides a kit for performing the protein recruitment system.
REFERENCES:
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Spencer et. al.. A general strategy for producing conditional alleles of Src-like tyrosine kinases. PNAS, USA. vol. 92:9505-9809, Oct. 1995.
Chardin, Pierre et al., "Human Sos1: A Guanine Nucleotide Exchange Factor for Ras that Binds to GRB2." Science 260:1338-1343 (1993).
Quilliam, Lawrence A. et al., "Membrane-targeting Potentiates Guanine Nucleotide Exchange Factor CDC25 and SOS1 Activation of Ras Transforming Activity." Proc. Natl. Acad. Sci. USA. 91:8512-8516 (1994).
Osborne, Mark A. et al., "The Yeast Tribrid System-Genetic Detection of Trans-Phosphorylated ITAM-SH2-Interactions." Bio/tech. 13:1474-1478 (1995).
Aronheim, Ami et al., "Membrane Targeting of the Nucleotide Exchange Factor Sos is Sufficient for Activating the Ras Signaling Pathway." Cell. 78:949-961 (1994).
Aronheim Ami
Elledge Stephen J.
Karin Michael
Baylor College of Medicine
Degen Nancy
Sandals William
The Regents of the University of California
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