Protein recovery

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification

Patent

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Details

530416, 530417, 530418, 530422, 530825, 435 68, C07K 328, C07K 322

Patent

active

046562559

ABSTRACT:
An improved process is described for recovering proteins from insoluble inclusion bodies produced in transformant microorganisms. Sidestream precipitates isolated from chromatography effluent are resolubilized in a denaturing agent and the resolubilized proteins are renatured either by direct dialysis into denaturant-free buffer or by dialysis into a weaker denaturing agent followed by dialysis into denaturant-free buffer.

REFERENCES:
patent: 4511502 (1985-04-01), Builder
patent: 4511503 (1985-04-01), Olson
patent: 4518526 (1985-05-01), Olson

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