Protein purification on immobilized metal affinity resins effect

Details Protein purification on immobilized metal affinity resins effect Protein purification on immobilized metal affinity resins effect

1989-04-14

1992-12-08

Moskowitz, Margaret

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

530413, 530414, 530415, 530416, 530417, 530418, 530420, 530427, 530395, 530419, C07K 1506, C07K 318, C07K 328

Patent

active

051699362

ABSTRACT:
A process for the purification of proteins from solutions containing contaminants of similar net charge and molecular weight is provided, comprising contacting a solution containing the desired protein with an immobilized metal affinity chromatography resin in a buffer containing a low concentration of a weak ligand for the chelant of the resin. The adsorbed protein is then eluted using a buffer having a high concentration of the same weak ligand, e.g., Tris. Particularly preferred features employ agarose-iminodiacetic acid resins having copper cations and are especially useful in obtaining preparations of homogeneous, stable rsT4 proteins.

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