Protein purification and detection methods

Details Protein purification and detection methods Protein purification and detection methods

2007-12-18

2007-12-18

Carlson, Karen Cochrane (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

C530S350000, C435S183000

Reexamination Certificate

active

10345574

ABSTRACT:
The present invention relates to methods for purifying and for detecting the presence of a protein. The invention employs a NorpA sequence and a PDZ1 domain. A protein tagged with a NorpA sequence can associate with PDZ1 domain. Similarly, a protein tagged with a PDZ1 domain can associate with a NorpA sequence. This interaction forms an aspect of the protein purification methods and protein detection methods of the present invention. Recombinant expression vectors and a protein purification solid phase are also disclosed, as well as protein detection and purification kits.

REFERENCES:
patent: 7087388 (2006-08-01), Zuker et al.
patent: WO 91/17239 (1991-11-01), None
van Huizen et al, The EMBO J., vol. 17, pp. 2285-2297, (1998).
Kimple, M. et al, The EMBO J., vol. 20(16), pp. 4414-4422, (2001).
Reina et al., Computer-aided design of a PDZ domain to recognize new target sequences,Nature Structural Biology—advance online publicationpp. 1-7 (Jun. 24, 2002).

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