Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2007-04-24
2007-04-24
Russel, Jeffrey Edwin (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C435S183000, C530S387100
Reexamination Certificate
active
10663483
ABSTRACT:
The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatograph (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.
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patent: 5652348 (1997-07-01), Burton et al.
patent: 2003/0229212 (2003-12-01), Fahrner et al.
patent: 2004/0018573 (2004-01-01), Power et al.
Guerrier et al. New method for the selective capture of antibodies under physiological conditions. Bioseparation. 2000, vol. 9, pp. 211-221.
Guerrier et al. A dual-mode approach to the selective separation of antibodies and their fragments. Journal of Chromatography B. 2001, vol. 755, pp. 37-46.
Fong Robin
Heng Meng H.
Genencor International Inc.
Russel Jeffrey Edwin
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