Protein purification

Details Protein purification Protein purification

2007-03-13

2009-12-01

Russel, Jeffrey E (Department: 1654)

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

C435S183000, C530S387100

Reexamination Certificate

active

07626004

ABSTRACT:
The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatography (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.

REFERENCES:
patent: 5411873 (1995-05-01), Adams et al.
patent: 5429950 (1995-07-01), Power et al.
patent: 5652348 (1997-07-01), Burton et al.
patent: 5679543 (1997-10-01), Lawlis
patent: 6255115 (2001-07-01), Beijersbergen et al.
patent: 7208585 (2007-04-01), Fong et al.
patent: 2003/0229212 (2003-12-01), Fahrner et al.
patent: 2004/0018573 (2004-01-01), Power et al.
U.S. Appl. No. 60/373,889, filed Apr. 18, 2002, Power, et al.
U.S. Appl. No. 60/411,540, filed Sep. 18, 2002, Wang, et al.
*Harkki et al,, BioTechnology, 7:596-603, 1989.
*Mellor et al., Gene,24:1-14, 1983.
*Penttilâ et al., Gene 61:155-164, 1987.
*Sibakov.et al., Eur. J. Biochem., 145:567-572, 1984.
*Ward, Proc, Embo-Alko Workshop on Molecular Biology of Filamentous Fungi, Helsinki, pp. 119.128, 1989.
Guerrier, L. et al. , “New method for the selective capture of antibodies under physiological conditions”,Bioseparation, 9:211-221, 2000.
Guerrier, L. et al., “A Dual Mode Approach to the Selective Separation of Antibodies and Their Fragments”,Journal of Chromatography B, 755:37-46, 2001.
Harkki, A., et al, “A Novel Fungal Expression System: Secretion of Active Calf Chymosin from the Filamentous FungusTrichoderma reesei”, BioTechnology, 7:596-603, 1989.
Iordanescu, S., “Recombinant Plasmid Obtained from Two Different, Compatible Staphylococcal Plasmids”,J. Bacteriol, 597-601, 1975, vol. 124.
Mellor, J. et al., “Efficient Synthesis of Enzymatically Active Calf Chymosin inSaccharomyces cerevisiae”, Gene, 24:1-14, 1983.
Penttilä, M. et al., “A Versatile Transformation System for the Cellulolytic Filamentous FungusTrichoderma reesei”, Gene61:155-164, 1987.
Sibakov, M. et al., “Isolation and the 5′-end nucleotide sequence ofBacillus licheniformisα-amylase gene”,Eur. J. Biochem., 145:567-572, 1984.
Ward, M., “Heterologous Gene Expression inAspergillus”, Proceedings of the EMBO-Alko Workshop on Molecular Biology of Filamentous Fungi, Helsinki, pp. 119-128, 1989.
Nickerson, Kenneth W. and Bulla, Lee A., Jr., “Lipid Metabolism During Bacterial Growth, Sporulation, and Germination: an Obligate Nutritional Requirement inBacillus thuringiensisfor Compounds That Stimulate Fatty Acid Synthesis,”Journal of Bacteriology, 123(2):598-603 (1975).

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