Protein purification

Details Protein purification Protein purification

1986-10-20

1993-01-12

Draper, Garnette D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530412, 530417, 530350, 435803, 435 696, 435 695, C07K 328, C07K 322, C07K 320

Patent

active

051791994

ABSTRACT:
A method of removing an organic solvent from a mixture that includes a compound having a polypeptide segment and the organic solvent, the method including the steps of contacting the mixture with an ion exchange resin under conditions that allow the compound to bond to the resin; and washing the resin with a first aqueous solution that elutes the organic solvent from the resin while allowing the compound to remain bound to the resin.

REFERENCES:
patent: 4405601 (1983-09-01), McEntire
patent: 4667016 (1987-05-01), Lai et al.
patent: 4675387 (1987-06-01), Korant
patent: 4734362 (1988-03-01), Hung et al.
patent: 4748234 (1988-05-01), Dorin et al.
patent: 4839419 (1989-06-01), Kraemer et al.
Light, BioTechniques, 3(4), 1985, pp. 298-306.
Sofer et al., BioTechniques, Nov./Dec. 1983, pp. 198-203.
Hodgkinson et al., (1981), Biochem. J., 199:619-27.

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