Protein-protein interactions and methods for identifying...

Details Protein-protein interactions and methods for identifying... Protein-protein interactions and methods for identifying...

2007-02-13

2007-02-13

Gabel, Gailene R. (Department: 1641)

Chemistry: analytical and immunological testing

Involving kinetic measurement of antigen-antibody reaction

C436S503000, C436S507000, C436S517000, C436S518000, C436S524000, C436S528000, C436S538000, C436S087000, C436S089000, C435S006120, C435S007100, C435S007200

Reexamination Certificate

active

10010310

ABSTRACT:
The invention relates to protein-protein interactions and methods for identifying interacting proteins and the amino acid sequence at the site of interaction. Using overlapping hexapeptides that encode for the entire amino acid sequences of the linker domains of human P-glycoprotein gene 1 and 3 (HP-gp1 and HP-gp3), a direct and specific binding between HP-gp1 and 3 linker domains and intracellular proteins was demonstrated. The method of the present invention was further validated with Annexin. The present invention thus demonstrates a novel concept whereby the interactions between two proteins are mediated by strings of few amino acids with high and repulsive binding energies, enabling the identification of high affinity binding sites between any interacting proteins.

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