Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-12-03
1996-03-12
Martinell, James
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 692, 435 697, 435 698, C12P 2100
Patent
active
054985293
ABSTRACT:
This invention relates to a process for producing a heterologous polypeptide in Streptomyces which involves transforming a Streptomyces organism with a recombinant DNA molecule comprising an LEP-10 of LTl sequence selected from the group consisting of a sequence encoding a gene expression unit, a promoter sequence or an export control encoding sequence each operatively linked to a heterologous coding sequence and then culturing the transformed Streptomyces such that the heterologous polypeptide is expressed.
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Helfman et al., Proc. Natl. Acad. Sci. USA 80: 31 (1983).
Verweij et al., Nucleic Acids Res. 13: 4699 (1985).
Young et al., Proc. Natl. Acad. Sci. USA 80: 1194 (1983).
Ikenaka et al., J. Biochem. 76: 1191 (1974).
Sugino et al., J. Biol. Chem. 253: 1546 (1978).
Bezborodov et al., Chem. Abstr. 102: 217993k (1985).
Berka Thomas R.
Fornwald James A.
Gorniak Joselina G.
Rosenberg Martin
Strickler James E.
Jervis Herbert H.
Lentz Edward T.
Martinell James
SmithKline Beckman Corporation
Suter Stuart R.
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