Protein profiling of hyper acidic plants and high protein...

Details Protein profiling of hyper acidic plants and high protein... Protein profiling of hyper acidic plants and high protein...

2005-05-17

2005-05-17

Leith, Patricia (Department: 1654)

Drug, bio-affecting and body treating compositions

Plant material or plant extract of undetermined constitution...

C514S002600

Reexamination Certificate

active

06893667

ABSTRACT:
The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargoniumsp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.

REFERENCES:
Arora et al. Water-Stress-Induced Heat Tolerance in Geranium Leaf Tissues: A Possible Linkage Through Stress Proteins? Physiologia Plantarum vol. 103, No. 1 pp. 24-34.

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