Chemistry: molecular biology and microbiology – Vector – per se
Patent
1993-12-08
1995-06-06
Martinell, James
Chemistry: molecular biology and microbiology
Vector, per se
435 691, 935 29, C12N 1570
Patent
active
054222753
ABSTRACT:
Methods and compositions are provided for producing heterologous proteins in a micrcorganism host, particularly bacterium, without fusion to an endogenous protein. Particularly, the heterologous gene may be inserted into a vector comprising an endogenous gene, where the heterologous gene is preceded by a ribosomal binding site. A heterologous enzyme functional in a bacterial host is demonstrated.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen
Alt, et al. (1978) Journal of Biological Chemistry 253:1357. Selective Multiplication of Dihydrofolate Reductase Genes in Methotrexate Resistant Variants of Cultured Murine Cells.
Backman and Ptashne (1978) Cell 13:65-72. Maximizing Gene Expression on a Plasmid Using Recombination In Vitro.
Villa-Komaroff et al, Proc. Natl. Acad. Sci. USA 75:3727 (1978).
Chang Annie C. Y.
Cohen Stanley N.
Nunberg Jack H.
Schimke Robert T.
Martinell James
Rowland Bertram I.
The Board of Trustees of the Leland Stanford Jr University
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