Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-05-13
1997-06-24
Ketter, James S.
Chemistry: molecular biology and microbiology
Spore forming or isolating process
4352404, 4353201, 43525411, C12N 506, C12N 508, C12N 1585
Patent
active
056416709
ABSTRACT:
The invention relates to constructs comprising: a) a targeting sequence; b) a regulatory sequence; c) an exon; and d) an unpaired splice-donor site. The invention further relates to a method of producing protein in vitro or in vivo comprising the homologous recombination of a construct as described above within a cell. The homologously recombinant cell is then maintained under conditions which will permit transcription and translation, resulting in protein expression. The present invention further relates to homologously recombinant cells, including primary, secondary, or immortalized vertebrate cells, methods of making the cells, methods of homologous recombination to produce fusion genes, methods of altering gene expression in the cells, and methods of making a protein in a cell employing the constructs of the invention.
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Heartlein Michael W.
Selden Richard F.
Treco Douglas A.
Ketter James S.
Transkaryotic Therapies Inc.
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