Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals
Patent
1990-08-15
1992-08-04
Kepplinger, Esther L.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
436175, 436501, 436 17, 436800, 436808, 436825, 435 71, 435961, 435962, 435975, G01N 33543, G01N 3353
Patent
active
051358751
ABSTRACT:
A precipitation reagent for use in analytical systems for the determination of hydrophobic analytes in a biological test sample, particularly analytical systems employing specific binding proteins for such analytes. The precipitation reagent precipitates interfering proteins, hemoglobin, and other interfering substances from a biological test sample while, at the same, maintaining hydrophobic analytes in solution and minimizing the denaturation of specific binding proteins, such as, for example, antibodies, which may be present in an immunoassay system. The precipitation reagent comprises a zinc salt, a glycol, and a straight or branced alcohol from about 1 to 4 carbon atoms, and may optionally contain an acid. A preferred precipitation reagent comprises zinc sulfate, methanol and ethylene glycol, and is particularly useful in a fluorescent polarization immunoassay for the determination of hydrophobic analytes, especially cyclosporine.
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Abstract, Soviet Union Patent 1109170 from World Patent Index (Aug. 23, 1984).
Meucci Victoria P.
Simpson Elizabeth A.
Zajac Mariola B.
Abbott Laboratories
Bidwell Carol E.
Breininger Thomas M.
Collins Daniel W.
Kepplinger Esther L.
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