Protein PP 15 preprared by genetic manipulation

Details Protein PP 15 preprared by genetic manipulation Protein PP 15 preprared by genetic manipulation

1992-08-11

1994-05-31

Fleisher, Mindy B.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, 4353201, 536 231, C07H 2102, C07H 2104, C12N 1570, C12N 1574

Patent

active

053170946

ABSTRACT:
Specific fragments of the protein PP15 were prepared by enzyme cleavage. Using statistical data, specific oligonucleotides were selected from the oligonucleotides coding for the specific PP15 fragments. These oligonucleotides were then used as probes to successfully screen a cDNA bank of mature human placenta and isolate the complete cDNA of PP15. The cDNA was sequenced and characterized. This cDNA can be used to prepare PP15 protein in prokaryotic or eukaryotic cells.

REFERENCES:
Maniatis et al., Molecular CLoning (1982) Cold Spring Harbor Press; CSH, N.Y. pp. 388-389.
Hames et al., Nucleic Acid Hyhidization, (1985) IRL Press, Wash. D.C. pp. 8-9.
Bloomfield et al., Physical Chemistry of Nucleic Acids (1974), Harper & Row NY, N.Y. pp. 328-332.
Barker et al. Eur J. Biochem 127:449-457 1982.
Grundmann et al. Nuc Acids Res 16(10):4721 (1988).
R. Lathe, J. Mol. Biol., 183 (1985) pp. 1-12.
Chirgwin et al., Bioichemistry, 18 (1979) pp. 5294-5299.
Aviv et al. Proc. Natl. Acad. Sci. USA, 69, No. 6 (1979) pp. 1408-1412.
Gubler et al., Gene 25 (1983) pp. 263-269.
T. Maniatis et al., Molecular Cloning, A Laboratory Manual, Cold Spring Harbour (1982), pp. 242-246.
Enquist et al., Methods in Enzymology, 68 (1979) pp. 281-298.

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