Chemistry: analytical and immunological testing – Process or composition for determination of physical state... – Surface area – porosity – imperfection – or alteration
Patent
1992-10-14
1994-06-21
Hill, Jr., Robert J.
Chemistry: analytical and immunological testing
Process or composition for determination of physical state...
Surface area, porosity, imperfection, or alteration
435 697, 4351727, 536 234, C12N 1509, C12N 1562
Patent
active
053228014
ABSTRACT:
A rapid, simple and inexpensive method to screen and classify proteins as partners of dimeric proteins is described. The method utilizes fusion protein constructs containing a DNA binding domain and complementary dimerization domains from a different protein. According to the method of the invention, protein partner heterodimer formation is detected by the ability of the protein partner to displace formation of DNA binding domain homodimers, and thus reveal a phenotypic change in a bacterial host which was dependent upon maintenance of the DNA binding domain homodimer configuration. The method of the invention may further be used to identify compounds of interest which inhibit such heterodimer formation, and especially to identify compounds which prevent heterodimer formation and activation of oncogenic transcriptional regulatory proteins.
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Bunker Christopher A.
Kingston Robert E.
Hill Jr. Robert J.
The General Hospital Corporation
Ulm John D.
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