Protein modification to provide enzyme activity

Details Protein modification to provide enzyme activity Protein modification to provide enzyme activity

1983-03-21

1987-12-15

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435195, 530402, C12N 900, C12N 914

Patent

active

047133350

ABSTRACT:
A naturally occurring protein is chemically modified to provide the protein with activity of a selected enzyme. The protein does not contain activity of the selected enzyme before modification. Modification is carried out by grossly denaturing the protein, partially renaturing the protein to form a partially denatured protein, contacting the partially denatured protein with an enzyme inhibitor of the selected enzyme, crosslinking the protein in the presence of the inhibitor and recovering a modified protein having activity of the selected enzyme.

REFERENCES:
patent: 4609925 (1986-09-01), Keyes et al.
Battelle Report, Verification of Semisynthetic Activity, Owens-Illinois, Jul. 14, 1981.
Yamauchi, et al., Reversible Conversion of Lysine Monooxygenase to an Oxidase, J. of Biol Chem., vol. 248, 1973, pp. 3750-3752.
Mahler et al., Biological Chemistry, 1966, Harper and Row, N.Y., pp. 287-295.
Beaven et al., International Journal of Peptide Research, vol. 5, pp. 215-218, 1973.

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