Protein modification to provide enzyme activity

Details Protein modification to provide enzyme activity Protein modification to provide enzyme activity

1980-12-04

1987-12-29

Naff, David M.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

435195, 530402, C12N 900, C12N 914

Patent

active

047161167

ABSTRACT:
A naturally occurring protein is chemically modified to provide the protein with activity of a selected enzyme. The protein does not contain activity of the selected enzyme before modification. Modification is carried out by partially denaturing the protein, contacting the partially denatured protein with an enzyme inhibitor of the selected enzyme, crosslinking the protein in the presence of the inhibitor and recovering a modified protein having activity of the selected enzyme.

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Beaven et al., Modification of the Enzymic Activity of Trypsin by Intramolecular Cross-links, Int. J. Peptide Protein Res., V5, 1973, pp. 215-218.
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Offord, Semisynthetic Proteins, Wiley and Sons, New York, 1980, p. 1-24.

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