Protein-mediated nuclear import of DNA

Details Protein-mediated nuclear import of DNA Protein-mediated nuclear import of DNA

1997-03-25

1998-11-03

Elliott, George C.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

C07K 14195

Patent

active

058310207

ABSTRACT:
The invention provides a nuclear targeting molecule having a nucleic acid sequence which encodes a nuclear localization signal of a VirE2 protein of Agrobacterium, the nuclear localization signal of the VirE2 protein having a mutation therein which renders the nuclear localization signal of the VirE2 protein capable of targeting deoxyribonucleic acid into the nucleus of an animal cell. The invention further provides a plasmid for targeting a DNA molecule into the nucleus of a host cell. The plasmid comprises the nuclear targeting molecule and a DNA molecule to be targeted to a nucleus. This plasmid of the subject invention can be introduced into various host cells, and the nuclear targeting molecule will target the DNA molecule to the nucleus of the host cell. Thus, the invention further provides a method of targeting a DNA molecule into the nucleus of a host cell. A purified nuclear targeting peptide of a VirE2 protein of Agrobacterium is also provided, the nuclear targeting peptide of the VirE2 protein having a mutation therein which renders the nuclear targeting peptide of the VirE2 protein capable of targeting deoxyribonucleic acid into the nucleus of an animal cell.

REFERENCES:
Guralnick et al. Transport of DNA into the nuclei of Xenopus oocytes by a modified VirE2 protein of Agrobacterium. The Plant Cell vol. 8 pp. 363-373, 1996.
Bukrinsky, M.I., et al., Proc Natl Acad Sci USA 89:6580-6584(1992).
Bukrinsky, M.I., et al., Nature 365:666-669(1993).
Citovsky, V., et al., Science 256(5065):1802-1805(Jun. 26, 1992).
Citovsky, V., and Zambryski, P., Annu Rev Microbiol 47:167-197(1993).
Citovsky, V., et al., Proc Natl Acad Sci USA 91(8):3210-3214(Apr. 12, 1994).
Citovsky, V., and Zambryski, P., Membr Prot Transport 1:39-57(1995).
Clever, J., et al., Proc Natl Acad Sci USA 88:7333-7337(1991).
Dowty, M.E., et al., Proc Natl Acad Sci USA 92:4572-4576(1995).
Escudero, J., et al., Proc Natl Acad Sci USA 92:230-234(1995).
Finlay, D.R., et al., J Cell Biol 104:189-200(1987).
Fullner, K.J., et al., Mol Gen Genet 245(6):704-715(Dec. 15, 1994).
Herrera-Estrella, A., et al., Proc Natl Acad Sci USA 87(24):9534-9537(Dec. 1990).
Herrera-Estrella, A., et al., EMBO J 7:4055-4062(1988).
Howard, E.A., et al., Cell 68(1):109-118(Jan. 10, 1992).
Howard, E.A., and Citovsky, V., BioEssays 12:103-108(1990).
Howard, E.A., et al., UCLA Symp Mol Cell Biol New Ser 129:1-11(1990).
Jasper, F. et al., Proc Natl Acad Sci USA 91(2):694-698(Jan. 18, 1994).
Kaneda, Y., et al., Science 243:375-378(1989).
Koukolikova-Nicola, Z., et al., J Bacteriol 175(3):723-731(Feb. 1993).
Rossi, L., et al., Mol Gen Genet 239(3):345-353(Jun. 1993).
Shurvinton, C.E., et al., Proc Natl Acad Sci USA 89(24):11837-11841(Dec. 15, 1992).
Tinland, B., et al., EMBO J 14(14):3585-3595(Jul. 17, 1995).
Tinland, B. et al., Proc Natl Acad Sci USA 89(16):7442-7446(Aug. 15, 1992).
von Schwedler, U., et al., Proc Natl Acad Sci USA 91:6992-6996(1994).
Zupan, J.R., and Zambryski, P., Plant Physiol 107:1041-1047(1995).

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