Protein ligand binding region mapping system

Details Protein ligand binding region mapping system Protein ligand binding region mapping system

1993-03-31

1995-11-07

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 9141, 4351723, 435 691, 4353201, 4352523, 935 77, 935 23, 935 79, 935 80, 436501, C12Q 168, C12N 1563, C12P 2100

Patent

active

054647452

ABSTRACT:
A method for determining the nucleic acid sequence that encodes ligand binding domain on any protein encoded by a known gene includes the steps of non-specifically cleaving the coding region of the known gene, size-selecting the cleavage products, cloning the size-selected gene products into an expression vector active in a heterologous host, using a novel cloning strategy expressing the peptides produced by the clones and screening for clones that produce peptides that interact with an antibody or ligand of interest.

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