Protein isolation

Details Protein isolation Protein isolation

2007-10-16

2007-10-16

Carlson, Karen Cochrane (Department: 1656)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

Reexamination Certificate

active

10921741

ABSTRACT:
A fusion protein containing a polyhedrin of a virus and a foreign polypeptide sequence. Also disclosed are a recombinant occlusion body containing the fusion protein, an isolated nucleic acid containing a sequence encoding the fusion protein, and a recombinant virus containing the nucleic acid. Within the scope of this invention are related expression vectors, host cells, compositions, and preparation methods.

REFERENCES:
patent: 4870023 (1989-09-01), Fraser et al.
patent: 5976552 (1999-11-01), Volvovitz
Ikeda et al., Characterizations of natural and induced polyhedrin gene mutants ofBombyx moricytoplasmic polyhedrosis viruses, Arch. Virol. (1998) 143: 241-248.
GenCore version 5.1.6, Result 1, pp. 1-7.
Verne A. Luckow et al. “Signals Important for High-Level Expression of Foreign Genes inAutographs californicaNuclear Polyhedrosis Virus Expression Vectors”, Virology 167:56-71, 1988.
J. H. McLinden et al. “Expression of foreign epitopes on recombinant occlusion bodies of baculovirus”. Vaccine 10(4):231-237, 1992.

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